RNAseq and scRNAseq analysis of UPFL mouse tumors

Fibroblast growth factor receptor 3 (FGFR3) is altered in up to 50% of urothelial cancers (UC), yet its functional impact is not fully understood. We generated a Cre-inducible FGFR3 S249C allele (LSL-FGFR3 S249C) to understand the functional impact of FGFR3 activation on UC biology, the immune microenvironment, and how FGFR inhibition combines with PD1 immune checkpoint inhibition (ICI). Uroplakin3a (Upk3a) driven expression of Trp53 R270H and FGFR3 S249C (UPFL) promoted tumor formation. Overall design: bulk RNAseq from primary UPFL tumors (n=7) and scRNAseq from primary UPFL control (n=2) or Erdafitinib (n=2) treated tumors. Pooled scraped urothelium from 10 wild-type female mice without treatment are anlyzed using scRNAseq