PyRom1(-) parasites progress normally through the mosquito vector.

a)Percent gametocytemia calculated per mouse used during mosquito feeding. Shown is the mean with standard deviation.b)Mosquito midguts dissected at day 8 post feeding. Mean number oocysts per midgut reported with Standard Error Mean.c)Number of midguts dissected and counted per parasite line for each experiment.d)Prevalence is calculated as the percentage of infected midguts over total midguts analyzed.e)Statistical analysis using non parametric 2-way ANOVA/Tukey's post test with Prism Graph Pad.f)Salivary glands dissected at day 14 post feeding. Mean number of sporozoites/mosquito is reported ± standard deviation.