Identification of TI-4-interacting proteins

Transcriptional co-activator with PDZ-binding motif (TAZ) (the official gene name; WWTR1) interacts with transcription factors in the nucleus but when phosphorylated, translocates to the cytoplasm and undergoes degradation. Chemical compounds that modulate the subcellular localization of TAZ are supposed to activate or inhibit TAZ. We identified a compound that shifts TAZ to the cytoplasm independently of phosphorylation and named it TI-4. We used affinity beads and obtained as a putative target of TI-4 Chromosomal Segregation 1 Like (CSE1L), which is involved in the recycling of importin alpha and is known as a biomarker of cancer malignancy.