Metatranscriptomic Analysis for Eukaryotic Functional Genes in Forest Soil

For the extraction of environmental RNA after enrichment with wheat bran and avicel, the sample was collected from the forest humic soil in the site of AIST. A forest soil in the site of AIST Tsukuba, Japan (36.06N 140.17E). The extraction of total RNA and the purification of polyadenylated eukaryotic mRNA from the enriched soil sample were used to commercially available kit, respectively. The synthesized cDNA sample from the purified mRNA was outsourced to Dragon Genomics Center for 454 GS FLX sequencing. The aim of this study is the exploration of the novel eukaryotic functional genes from an environmental sample using metatranscriptomic approach. This method is widely applicable to the provision of novel eukaryotic enzymes and proteins of potential industrial or medical use.