Dynamic Shifts in the Composition of Resident and Recruited Macrophages Influence Tissue Remodeling in NASH. Dynamic Shifts in the Composition of Resident and Recruited Macrophages Influence Tissue Remodeling in NASH
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA682557
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资源简介：
NASH is associated with signficant changes in liver macrophage composition. To gain insight into this heterogenity we performed scRNA on liver myeloid cells from mice fed a HFD for 16 weeks. Overall design: Three C57CL/6 male mice were fed High fat, high sucrose diet (Research Diets -cat# D12492) for 16 weeks after which immune cells were isolated from the liver using portal vein perfusion and a percoll gradient protocol. The cells underwent flow sorting on live singlet cells and were gated on CD11bhi, F4/80int and CD11bint, F4/80hi populations. Eosinophils and neutrophils were removed using Ly6G and siglecF exlusion. Samples of these macrophages were mixed 1:1 and the 3 mouse samples were pooled to create a signal sample for scRNA seq.
创建时间：
2020-12-04
