Identification of LIN-29 target genes

To further explore the regulatory network coordinated by LIN-29, and to possibly uncover potential new roles for this transcription factor, we searched for its downstream target genes at the genomic level. To do this, we chose not to modify LIN-29 expression via loss-of-function mutations in upstream heterochronic pathway components, but rather to temporally misexpress LIN-29 directly and examine changes in development and in gene expression. We carried out early overexpression of LIN-29 by heat-shock inducing hs::lin-29 and hs::control strains in early L3 stage larva, and assessed gene expression by RNA-seq.