Comparing PBMCs from patients with different pruritic conditions

Chronic pruritis is a common and debilitating symptom that can manifest in multiple conditions. The goal of this study was to examine populations of circulating immune cells at the single cell level from patients with different pruritic conditions and compare them to healthy controls to identify possible biomarkers or therapeutic targets for chronic pruritis. Overall design: After giving informed consent, patients with moderate to severe atopic dermatitis, chronic pruritis of unknown origin also known as chronic idiopathic pruritis, cutaneous T cell lymphoma, or healthy controls provided whole blood samples. Peripheral blood mononuclear cells were isolated from whole blood and the 10X Genomics Chromium platform was used to generate single cell RNA-sequencing libraries, which were sequenced on an Illumina NovaSeq 6000.