Microtubules play important roles in organelle transport, the maintenance of cell polarity and chromosome segregation and generally form bundles during these processes. The fission yeast gene scp3+ wa
• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules. • The buffer used was PEM (also known as BRB80) with the inclusion of 5528mM D2O. • Images were taken with an And
Fibroblast data is shown for GFP-tau [33]. Ensconsin turnover was measured by expression of its microtubule-binding domain fused to 5 GFPs [37]. EB1-GFP turnover on microtubule ends was measured in bo
• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules. • The buffer used was PEM (also known as BRB80) with the inclusion of 16551mM D2O. • Images were taken with an An
