Expression data from untreated and irradiated Nipa WT and Nipa KO hematopoietic stem cells (HSCs). Expression data from untreated and irradiated Nipa WT and Nipa KO hematopoietic stem cells (HSCs)

The F-Box-Protein NIPA might define a novel regulator of aging and critical stress response in hematopoietic stem cells To investigate the underlying mechanisms and activated pathways of NIPA-mediated HSC function in steady state and under stress conditions, we performed Affimetrix-based global gene expression analysis on untreated and irradiated, aged Nipa WT and Nipa KO LSK (Lineage-, SCA1+, cKIT+) cells and identified different expression profiles depending on NIPA Overall design: LSKs out of 3 aged Nipa WT or Nipa KO mice were merged to one sample, cultured in BM media, either not treated or 4 Gray irradiated and harvested 3 hours later for RNA extraction and Affimetrix-based global gene expression analysis.