Homo sapiens Raw sequence reads

Our study focuses on gliomas, the most common primary brain tumors in adults, and understanding the role of endogenous transposable elements in these malignancies. Using genomic DNA from 7 low passage oncosphere cell lines derived from primary GBM biopsies, 3 secondary GBM tissue samples, and matched normal blood samples, we performed transposon insertion profiling by deep sequencing (TIPseq) of the active subfamily of Long INterspered Element-1 (LINE-1) elements to find evidence of somatic retrotransposition. Our findings indicate that although LINE-1 can act as an infrequent insertional mutagen in secondary glioblastomas, retrotransposition is uncommon in these central nervous system tumors as compared to other neoplasias.