Ip, Wallace (2010) CIL:6567, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were fixed in buffered glutaraldehyde, treated with tannic acid and quick-frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

This image was recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:6564.

兔类腰大肌纤维在甘油溶液中维持恒定长度，并通过甘油浸渍诱导僵直。随后，纤维经缓冲戊二醛固定，用单宁酸处理，并在Heuser型冷冻装置中通过接触液氦冷却的铜块进行快速冷冻，随后在Balzers 400冷冻断裂机中进行冷冻断裂。样品随后在24度角下进行Pt-C旋转复制，并在100 keV工作电压的JEOL 100CX2透射电子显微镜下进行观察。图像记录在柯达4489胶片上，放大倍数为75,000倍。在+5度和-5度处拍摄了立体对。胶片使用尼康Coolscan 9000ED扫描仪以20微米像素间距进行扫描。与CIL:6564相关的立体对。