Hazardous effects of 6PPD on human small intestinal organoids

N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is the most ubiquitously utilized antioxidant in the manufacture of rubber products. While it has been associated with the development of human colorectal cancer (CRC), its intestinal hazardousness in humans remains experimentally unconfirmed. In this study, human small intestinal organoids were used to assess the intestinal toxicity of 6PPD. The findings of this study demonstrated that exposure to 6PPD at concentrations ranging from 300 to 900 μg·L⁻¹ resulted in impaired organoid growth in a concentration-dependent manner, as evidenced by reduced organoid count and size. Although even the highest concentration tested (900 μg·L⁻¹) did not induce overt cell death, viability was significantly inhibited across this concentration range, as evidenced by decreased Calcein fluorescence and CCK-8 metabolic activity. Immunofluorescence analysis further revealed suppressed proliferation and downregulation of epithelial and goblet cell mark..., , # Hazardous effects of 6PPD on human small intestinal organoids

Dataset DOI: [10.5061/dryad.jwstqjqpd](https://doi.org/10.5061/dryad.jwstqjqpd)

## Description of the data and file structure

### File: original_images__data_and_description.zip

The data for the four figures in the manuscript (Figures 1-4) are organized into separate folders labeled F1 through F4, respectively. Each folder contains the original images and processed data used to create its corresponding figure. Please note that the folder prefixes 'F' and 'D' have distinct meanings: F1-F4 refer to the figure number, while D1-D4 refer to the experimental day (e.g., Day 1). For each folder, the image counts and relevant statistical data are recorded in a CSV file within that same folder.

Folder F1 contains data related to the culture and 6PPD treatment of human small intestinal organoids. Folder F1A includes images of human small intestine organoids thawed from cryopreservation and cultured under standard conditions. Imag...,