NAT10-mediated ac4C-modification exacerbates ferroptosis by stabilizing HMOX1 in Deep vein thrombosis [acRIP-Seq]

In our study, we aimed to identify the mechanisms closely related to ac4C. Through acRIP-seq analysis, we found that NAT10 enhances the stability of HMOX1 via ac4C modification, which leads to iron overload and lipid peroxides. This forms a positive feedback loop that exacerbates DVT. Overall design: HUVECs were transfected with NC or si-NAT10.The RNA is first extracted from the sample (extracted using TRIzol), the RNA is quality controlled, and the concentration and purity of the RNA is detected. Total RNA was subjected to immunoprecipitation according the manufacturer's instructions. The samples were sequenced using a NovaSeq platform (Illumina).