Lactiplantibacillus Plantarum 124 Alleviates Sleep Depriva-tion Induced Intestinal Barrier Damage in Mice via Regulating the Gut Microbiota

Intestinal diseases caused by sleep deprivation (SD) are severe public health threats worldwide. However, whether probiotics attenuates the intestinal damage induced by SD remains unclear. This study, we used fecal microbiota transplantation and antibiotic pretreatment to investigate the protective role of Lactiplantibacillus plantarum (L. plantarum) 124 against SD-induced intestinal barrier damage in C57BL/6 mice. Compared with the normal sleeping mouse, we observed a de-crease in antioxidant ability, down-regulation of anti-inflammatory cytokines, and up-regulation of pro-inflammatory cytokines in sleep deprivation mice. This resulted in decreased tight junction protein expression and increased intestinal barrier permeability. In contrast, intragastric admin-istration with L. plantarum 124 reversed SD-induced intestinal oxidative stress, inflammation, co-lonic barrier damage, and dysbiosis of the microbiota in the colon. In addition, L. plantarum 124 restored gut microbiota homeostasis via restoring abundance, including Dubosiella, Faecalibaculum, Bacillus, Lachnoclostridium, and Bifidobacterium. Further studies showed that gut microbiota medi-ated the SD-induced intestinal damage and the treatment of L. plantarum 124 on SD-induced co-lonic barrier damage. L. plantarum 124 is a potential candidate for alleviating SD-induced intestinal barrier damage. Overall, L. plantarum 124 consumption attenuates intestinal oxidative stress, in-flammation, and intestinal barrier damage in SD-induced mice via the modulation of gut microbes.