Transcriptional profiling of microglia in the injured brain reveals distinct molecular features underlying neurodegeneration. Transcriptional profiling of microglia in the injured brain reveals distinct molecular features underlying neurodegeneration

We applied whole transcriptome analysis of microglia acutely isolated at different timepoints after a cortical stab wound injury to gain insight into genes that might be dysregulated and transcriptionally different between males and females after cortical injury. Overall design: Total RNA was extracted from the FACS-sorted microglia that collected from freshly dissociated cortex at the site of cortical stab wound injury or from the equivalent site at the contralateral non-injured hemisphere of adult Cx3cr1CreER mice on days 1, 4, 7 and 14 following stab wound injury, in two biological replicates, using Illumina Hiseq4000.