Multi-omics analysis reveals altered lipid metabolism in the skin of high fat diet-fed mice of atopic dermatitis

To identify the obesity-induced exacerbating mechanisms of AD symptoms by multi-omics analysis. Then, we demonstrated the therapeutic effect of GHJGS in circulating lipid profiles. Overall design: For diet-induced obesity, mice were fed a HF diet (60 kcal% fat, D12492, Research Diets, New Brunswick, NJ) for up to 4 weeks before the induction of AD. Sham groups were fed a normal autoclave rodent chow (38057, Purina Korea, Seoul, Korea). After the induction of obesity for 4 weeks, (1) the sham [normal diet + vehicle + distilled water (DW)]; (2) AD [normal diet + 2,4-dinitrochlorobenzene (DNCB) + DW]; (3) HF+AD (HF diet + DNCB + DW) (4) HF+AD+GHJGS; HF diet + DNCB+ GHJGS 617 mg/kg/day) for 6 weeks. To induce AD, dorsal skin was applied three times a week with 0.5% DNCB mixed in acetone/olive oil (3:1).