<b>A high-quality reference genome assembly of </b><b>Prinsepia uniflora</b><b>(Rosaceae)</b>

In this study, we presented a high-quality, chromosome-levelgenome assembly of a traditional Chinese medicinal plant, Prinsepia uniflora. The final assembly spans 1,272.71 Mb across 16 pseudochromosomes, with contig and super-scaffold N50 values of 2.77 and 79.32 Mb, respectively. A total of 875.99 Mb of repetitive sequences were annotated, with transposable elements spanning 777.28 Mb or 61.07% of the entire genome. We predicted 49,261 protein-coding genes within the repeat-masked assembly, 45,256 (91.87%) were functionally annotated, 5,127 (10.41%) were tandemly duplicated, and 2,373 (4.82%) were identified as transcription factor genes. In addition, we identified 3,080 non-coding RNAs spanning 0.51 Mb genome sequences. The reference-level P. uniflora genome assembly and annotation presented here will be highly useful indeep mining of genomic information of P. uniflora, and beneficial for comparative genomics and evolutionary studies of Rosaceae species.本研究报道了传统药用植物蕤核（Prinsepia uniflora）的高质量染色体级基因组组装结果。最终组装序列总长1272.71 Mb，分布于16条假染色体上；其重叠群（contig）N50与超支架（super-scaffold）N50值分别为2.77 Mb与79.32 Mb。本研究共注释得到875.99 Mb的重复序列，其中转座因子序列总长777.28 Mb，占全基因组的61.07%。在屏蔽重复序列的组装序列中，我们共预测得到49261个蛋白编码基因，其中45256个（占比91.87%）获得了功能注释，5127个（占比10.41%）为串联重复基因，2373个（占比4.82%）被鉴定为转录因子基因。此外，我们还鉴定出3080条非编码RNA（non-coding RNAs），其覆盖的基因组序列总长0.51 Mb。本研究报道的参考级蕤核基因组组装与注释结果，将为蕤核基因组信息的深度挖掘、蔷薇科物种的比较基因组学与进化研究提供重要支撑。