RNA-seq analysis of alternative splicing between two Arabidopsis accessions and its inheritance in hybrid

To uncover the possible regulatory mechanism in regulatory alternative splicing in plants evolution, we used RNA-seq to compare the transcriptomes in Arabidopsis ecotypes Col and C24. We found different class of AS types have different regulatory mechanisms in Arabidopsis and divergence of alternative splicing in different class is varied in evolution. Sequence variation analysis had found evolution divergent AS events significantly altered their sequences compared to conserved events and the variation is AS class specific. Overall design: RNA-seq analysis of alternative splicing in the shoots of 14 day old seedlings of two Arabidopsis(Col and C24) and their reciprocal hybrids (Col x C24 and C24 x Col).