New insights of bacteria species associated with bronchiectasis using 16S rDNA pyrosequencing

Background To test the efficiency of the 16S rDNA sequencing approach for the pathogen tracing in bronchitis patients and research the complexity of the flora associated with bronchiectasis. Methods Broncho alveolar lavage fluid(BALF) were collected from patient with bronchiectasis, and sent to the laboratory for culture and sequencing immediately. Then we conducted metagenomic analysis using 16S rDNA pyrosequencing data. Results OUT analysis showed the composition of the bacteria community in sample was complex but the bacteria of P. aeruginosa (51.3%) took the main part as same as culture. Other reads were identified to Carnobacterium sp. (9.3%), Serratia proteamaculans (1.5%), Enterobacter cloacae (1.2%), et al. Conclusions The detection with 16S rDNA pyrosequencing technology is more sensitive than rountine culture, and can detect the co-infection or symbiosis in airway. The detection with 16S rDNA pyrosequencing technology gives us a novel and convenient way to look into the complex composition of bacterial communities in bronchiectasis.