Detaching cells from human iPSc-derived thymic organoids (hTOs) seeded with primary early thymic progenitors (ETPs) at late phase of maturation (D28)

We have employed a single-cell sequencing approach using 10x Genomics scRNA-seq to study hematopoietic cell generation from hTOs (at the late phase of differentiation/maturation at D28) obtained through reaggreagation of iPSc-derived thymic epithelial progenitors (TEPs) with primary ETPs. Overall design: At D28 of organoid formation, cells were harvested by soft mechanical dissociation to obtain the CD45+ cell fraction and analyzed using scRNA-seq