Frequency of sectored colonies in unirradiated and UV-irradiated wild-type and <i>rad14</i> strains.<sup>1</sup>
收藏资源简介:
1As discussed in the text, red/white sectored colonies result from mitotic crossovers between SUP4-o and the centromere. The diploids used in our analysis had SUP4-o located near the left end of chromosome V or the right end of chromosome IV. 2Data from Lee et al. (2009). 3The selective method used in Lee et al. (2009) prevented the growth of cells without a recombination event on chromosome V. 4Data from Yin and Petes (2013). For the samples irradiated with 15 J/m2, we included only those samples grown in the absence of canavanine, and incubated at 300 following irradiation. 5Data from St. Charles and Petes (2013). 6As described in the text, most of the pink/red/white colonies in the wild-type strains reflect the plating of two adjacent cells, one with a crossover and one with unassociated events. 7Relative to the unirradiated wild-type strain PG311, the increase is 4309-fold. 8Relative to the unirradiated wild-type strain JSC25, the increase is 986-fold. Note that the unirradiated rad14 strains YYy37.6 and YYy37.8 are hyper-Rec. Frequency of sectored colonies in unirradiated and UV-irradiated wild-type and rad14 strains.1



