Additional file 2 of SEB genotyping: SmartAmp-Eprimer binary code genotyping for complex, highly variable targets applied to HBV

Additional file 2: Consensus sequence alignments results from various tools. We compared the alignment results of Kalign, MAFFT (8 consensus sequences) and Jalview/MAFFT (over 20,000 sequences). Aligning the 8 HBV genotypes before creating a consensus avoided bias towards the more common genotypes (C and B) and excluded the sequences with long insertions (highlighted in green).