Transcriptomic profiling of E.coli upon toxin expression

This project involves performing RNA seq on E.coli to characterize the transcriptomic responses triggered by the expression of the toxin. The main goal was to identify genes that become significantly differentially expressed upon toxin induction, providing insights into the molecular pathways affected by toxin activity. Analyzing these transcriptomic changes can help uncover the regulatory mechanisms the bacteria employs in response to toxin induced stress, potentially shedding light on bacterial stress responses, survival strategies, and the broader biological significance of the toxin under investigation.