A global transcriptome analysis of human epidermal keratinocytes upon inhibition of lncRNA WAKMAR1. A global transcriptome analysis of human epidermal keratinocytes upon inhibition of lncRNA WAKMAR1

Recent study has revealed that long non-coding RNAs (lncRNAs) perform as important regulators of cellular physiology and pathology, which makes them promising therapeutic and diagnostic entities. We found lncRNA WAKMAR1 is significantly down-regulated in wound-edge keratinocytes from venous ulcer and diabetic foot ulcer compared to the normal wounds. To study the genes regulated by WAKMAR1, we transfected lncRNA GapmeRs into human primary epidermal keratinocytes to inhibit its expression. We performed a global transcriptome analysis of keratinocytes upon inhibition of WAKMAR1 using Affymetrix arrays. We performed a global transcriptome analysis of keratinocytes upon inhibition of WAKMAR1 using Affymetrix arrays. Overall design: Expression profiling of primary human epidermal keratinocytes transfected with 20nM WAKMAR1 GapmeRs (GapmeR-WAKMAR1) 20nM control oligos (GapmeR-Ctrl) for 24 hours (biological triplicates in each group) was performed using Affymetrix GeneTitan system.