NICD2 overexpression in the bone marrow stromal cell line ST-2 cells

Notch signaling critically controls cell fate decisions in mammals, both during embryogenesis and in adults. In the skeleton, Notch suppresses osteoblast differentiation and sustains bone marrow mesenchymal progenitors during postnatal life. Stabilizing mutations of Notch2 cause the Hajdu-Cheney syndrome characterized by early onset osteoporosis in humans, but the mechanism whereby Notch inhibits bone accretion is not fully understood.To gain further insights about the mechanism we performed RNA-seq experiments with the doxycycline-inducible NICD2-ST2 cells with or without doxycycline treatment for 24 hrs.