Modifications of histone H3.1 and H3.3
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https://www.omicsdi.org/dataset/pride/PXD072852
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Nuclei were isolated from leaves of transgenic plants expressing HA-tagged H3.1 (HTR5) or H3.3 (HTR13). MNase treated nuclear extracts were used for immunoprecipitation with HA agarose beads (Roche). Immunoprecipitated proteins were analysed by 4-20% SDS-PAGE, silver stained, and protein bands corresponding to transgenic copy of H3 and endogenous H3 were used for trypsin and LysC digestion followed by LC-MS/MS.
创建时间：
2026-01-27
