Transcriptome response of metallicolous and a non-metallicolous ecotypes of Noccaea goesingensis to nickel excess

Root transcriptomic profile was comparatively studied in a serpentine (TM) and a non-metallicolous (NTM) population of Noccaea goesingensis in order to investigate possible features of Ni hyperaccumulation. The exposure to Ni altered the expression of 403 and 534 probe sets in roots of NTM and TM ecotypes respectively. Only 19 genes were commonly regulated in roots of both ecotypes. Plants of non-metallicolous population (NTM) and from serpentinite area (TM) were grown in control media and under Ni exposure. The treatment with Ni lasted three weeks, after this time plants were harvested and weighed. 15 plants from each experimental group were frozen in liquid nitrogen for RNA extraction. Total RNA Mini Kit (Bio-Rad, USA) was used for total RNA extraction from roots (from 5 plants per sample). Sequencing was conducted with the use of Ion PI Hi‐Q Sequencing 200 Kit (ThermoFisher Scientific, USA) and Ion PI Chip Kit v3 (ThermoFisher Scientific, USA) on the Ion Proton System (ThermoFisher Scientific, USA) according to standard protocol provided by the manufacturer.