RNA sequencing on EphrinB2 overexpression cardiac fibroblasts and vector cardiac fibroblasts within or without induction of osteogenic differentiation in the 7th day.. RNA sequencing on EphrinB2 overexpression cardiac fibroblasts and vector cardiac fibroblasts within or without induction of osteogenic differentiation in the 7th day.

Purpose: To investigate the function of EphrinB2 in the regulation of osteogenic differentiation of cardiac fibroblasts, we established EphrinB2-overexpressing cardiac fibroblasts and corresponding control group by lentivirus.Methods:Adult cardiac fibroblasts were isolated from 6 to 8-week-aged C57BL/6 mice, passage 1 cardiac fibroblasts plated at a density of 2.5 x 104 cells/cm2 were infected with the EphrinB2-overexpressing or vector lentivirus at an MOI of 20 for 12h, after 48 hours’ infection, osteogenesis was induced using differentiation medium (α-MEM supplied with 10% FBS, 10 mM β-glycerophosphate, 100nM dexamethasone and 50 µg/mL L-ascorbic acid). Cardiac fibroblasts harvested at Day7 were used for RNA sequencing. Overall design: Comparative gene expression profiling analysis of RNA-seq data for EphrinB2-overexpressing cardiac fibroblasts and vector cardiac fibroblasts within or without induction of osteogenic differentiation