Acetylation-specific interference by anti histone H3K9ac intrabody results in precise modulation of gene expression

The goal of this study is to compared the global gene expression changes driven by the histone PTM-selective interference with those induced by the inhibition of the corresponding modifying enzymes. We chose site-specific histone H3 acetylation as a target for this comparison, and we investigated the transcriptomic response in yeast cells, either expressing the intrabody anti-H3K9ac scFv-58F or treated with HATs inhibitors (Curcumin and CPTH2)