RNA-seq of Mycobacterium smegmatis mc2-155 SMR5 wild-type vs. a pafBC deletion strain under different growth/stress conditions. RNA-seq of Mycobacterium smegmatis mc2-155 SMR5 wild-type vs. a pafBC deletion strain under different growth/stress conditions

The goal of the experiment is to obtain a differential gene expression profile for Mycobacterium smegmatis mc2-155 SMR5 (Msm) wild-type in comparison to a pafBC deletion strain (MSMEG_3888 and MSMEG_3889). In addition, the gene expression is to be analyzed with respect to different growth/stress conditions. Four groups were etablished: (1) Msm wild-type under standard conditions (2) Msm delta-pafBC under standard conditions (3) Msm wild-type treated with mitomycin C (4) Msm delta-pafBC treated with mitomycin C.