Host response to plasmid replication and maintenance

Plasmids are widely used across molecular biology and are becoming increasingly valuable products, but robust plasmid replication is held back by stability issues in the host. This study investigated how Escherichia coli responds to plasmid stress at the transcriptional level by modulating plasmid copy number, plasmid size, selection marker and carbon source. This GEO contains controls from this study alone. RNA-seq profiling of Escherichia coli hosts containing plasmids of variable copy number, size and selection marker, and grown on either glucose or glycerol media.