Transcripts significantly differentially expressed in spleen leukocytes between 2 weeks and 4 weeks of age in NOD mice but not in two control strains.

Statistical analysis of mRNA expression data by unpaired t-Test (p<0.001) followed by Venn diagram analysis identified 66 probe sets (representing 65 different genes) that were uniquely differentially expressed in spleen leukocytes of NOD mice between 2 and 4 weeks of age in comparison to two control strains, NON and C57BL/6. Fold change (FC) was calculated by ratio of means of expression at 2 weeks versus 4 weeks; FC of genes of lower expression at 4 weeks (compared to 2 weeks) are indicated by negative values. Genes with FC<1.5 (and their FC values) included: Syt11 (1.47), Snap47 (1.46), Cklf (−1.45), Cd274 (1.43), Heatr7a (1.42), Fam76b (−1.41), Npsr1 (1.39), Nrn1 (1.38), Eef2k (1.24), Tomm34 (1.21), and IL17rb (1.2). Expression of approximately 62% (40) of the genes was upregulated at 4 weeks compared to expression at 2 weeks of age.