Genome wide CRISPR screen for BET inhibitor resisatnce in OCI-AML2 cells using Y. Kosuke library
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下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE159689
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资源简介：
OCI-AML2 BETi-naive or JQ1 resistant cells were infected with CRISPR/Cas9 library, selected for puromycin resistance for integration events and exposed to JQ1/CPI-0610 or vehicle, DMSO, at 200nanoMolar for JQ1 and 500nanoMolar CPI-0610 concentrations. Cells were collected at time 0 (post puromycin selection), day 14 JQ1 and 21 CPI-0610 , DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using highthroughput Illumina platform Hiseq using 6 samples per lane. Performed comparisons of sgRNA read counts between DMSO and BETi (JQ1 or CPI-0610) treated cells at corresponding time points.
创建时间：
2023-10-13
