Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions

This data corresponds to the following paper:

Title: Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions
DOI: 10.1126/sciadv.add3643
Journal: Science Advances
Authors: Josh P. Peters, Arne Brahms, Vivian Janicaud, Maria Anikeeva, Eva Peschke, Frowin Ellermann, Arianna Ferrari, Dana Hellmold, Janka Held-Feindt, Na-mi Kim, Johannes Meiser, Konrad Aden, Rainer Herges, Jan-Bernd Hövener, Andrey N. Pravdivtsev

In the upload the figures 2 - 6 are covered, so all figures from the main manuscript with acquired data.

Original abstract for the manuscript:

Dissolution dynamic nuclear polarization (dDNP) increases the sensitivity of magnetic resonance imaging by more than 10,000 times enabling in vivo metabolic imaging can be performed non-invasively in real-time. Here, we are developing a group of dDNP polarized tracers based on nicotinamide (NAM). We synthesized 1-15N-NAM and 1-15N nicotinic acid and hyperpolarized them with dDNP, reaching (13.0±1.9)% 15N polarization. We found that the lifetime of hyperpolarized 1-15N-NAM is strongly field- and pH-dependent, with T1 being as long as 41 s at a pH of 12 and 1 T while as short as a few seconds at neutral pH and fields below 1 T. The remarkably short 1-15N lifetime at low magnetic fields and neutral pH drove us to establish a unique pH neutralization procedure. Using 15N dDNP and an inexpensive rodent imaging probe designed in-house, we acquired a 15N MRI of 1-15N-NAM (previously hyperpolarized for more than an hour) in less than one second.