Single nuclei chromatin accessibility profiling in the midbrain of Park7-/- mice

We have performed single nuclei chromatin profiling of in vivo midbrain sections from 8-month old male mice depleted for Park7, a frequently mutated gene in early-onset Parkinson's disease (PD). The aim of this study was to identify the upstream regulatory events and key transcription factors acting at the chromatin level in the different cell types of midbrain, and thereby to understand the early molecular changes in this PD mouse model. This process included nuclei extraction before performing snATAC-seq