Expression analysis of TIS108-responsive genes in rice, IAC-165. Oryza sativa Japonica Group strain:IAC-165

Strigolactones (SLs) are known as multifunctional chemicals: plant hormone, inducer of hyphal branching in arbuscular mycorrhizal fungi and germination stimulant for Striga and Orobanche. TIS108 is triazole-containing chemical that reduces the endogenous level of a part of SLs in rice. While TIS108-treated rice did not show the accelerated outgrowth of tillers that is characteristic phenotype in SL-deficient mutants, TIS108 treatment effectively reduced the Striga infection in pot experiment. In this study, to estimate the effect of TIS108 on rice gene expression, we performed the RNA-seq analysis using the mRNA extracted from two-week-old rice roots with or without 10 µM TIS108 treatment.