Effect of thalamic deletion of mouse Sox2, or Nr2f1, on gene expression in the visual thalamus (dorsolateral geniculate nucleus, dLGN)

Effect of thalamic deletion of mouse Sox2, or Nr2f1, on gene expression in the visual thalamus (dorsolateral geniculate nucleus, dLGN), as described in "SOX2 and NR2F1 coordinate the gene expression program of the early postnatal visual thalamus" (bioRxiv doi number to be updated) To address the effect of Sox2, or Nr2f1, Cre-mediated thalamic deletion in mouse, on gene expression in the visual thalamus (dLGN), we dissected the dLGN from mutant (Sox2, or Nr2f1) and control mice at postnatal day 0 (P0), for both Sox2 and Nr2f1 mutants. We then performed gene expression analysis by RNAseq. Overall design: We dissected the dLGN from three pools of three mutant mice each, and three pools of three control mice each, for both Sox2 and Nr2f1 mutants. RNA from snap-frozen dLGNs was extracted with the RNeasy Micro Kit (QIAGEN), as described in detail in the paper, and