The number of recombinant colonies for each transformation was counted. Restriction-ligation was performed with continuous incubation at 37°C or for 20–50 cycles (2 min 37°C+5 min16°C). Cloning for pR

NIAID Data Ecosystem30

Plasmid length, insert length and cloning efficiency of the 10 test cases.

分子克隆效率

基因工程优化

aThe sum of insert length and template plasmid length does not automatically equal the product length since some inserts replace parts of the template. bNumber of colonies obtained in a single cloning

NIAID Data Ecosystem20

Data_Sheet_1_Escherichia coli BW25113 Competent Cells Prepared Using a Simple Chemical Method Have Unmatched Transformation and Cloning Efficiencies.pdf

分子克隆效率

细菌转化方法

Escherichia coli recA− strains are usually used for cloning to prevent insert instability via RecA-dependent recombination. Here, we report that E. coli BW25113 (recA+) competent cells prepared by usi

NIAID Data Ecosystem40

Efficiency of cloning in pWXY1.0 under different conditions.

分子克隆效率

载体构建优化

All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated at 37°C. Experiments were performed using 50 ng vector, and the corresponding amount of insert DNA at

NIAID Data Ecosystem30

Efficiency of the miRNA cassette subcloning into pKK-RNAi vectors.

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