CryoVirusDB: An Expert Labelled Cryo-EM Image Dataset for AI-Driven Virus Particle recognition and Extraction

With the advancements in instrumentation, image processing algorithms, and computational capabilities, single-particle electron cryo-microscopy (cryo-EM) has achieved nearly atomic resolutions in the 3D reconstruction of viruses. These detailed structures play a crucial role in comprehending the biological functions and advancing the development of more precise vaccines and antiviral treatments. Despite the effectiveness of deep learning in analyzing microscopic images, its potential in identifying and extracting virus particles from cryo-EM micrographs has been hindered by the limited availability of diverse and high-quality datasets. In this study, we introduce 'CryoVirusDB,' a labeled dataset containing coordinates of accurately selected virus particles in cryo-EM micrographs. CryoVirusDB comprises 9,941 micrographs featuring 9 different viruses along with the coordinates of 0.2 million virus particles in total. We anticipate that CryoVirusDB will enhance the capabilities of deep learning in accurately identifying virus particles in cryo-EM micrographs, thereby facilitating the subsequent 2D-3D reconstruction process. Instructions to download and use dataset: https://github.com/BioinfoMachineLearning/CryoVirusDB