File S1 - Modulating Human Mesenchymal Stem Cell Plasticity Using Micropatterning Technique

Supporting files. Figure S1, hMSCs were cultured on the cover glasses coated with 20 µm wide fibronectin printed pattern and evenly distributed fibronectin separately. Figure S2, Immunofluorescence staining of cardiac troponin T (cTnT), RUNX2 and PPARγ after 2 weeks of cell culture in normal growth medium. Figure S3, Immunofluorescence staining of cardiac troponin T and RUNX2 after 21 days of hMSCs culture (14 days in normal growth medium +7 days in osteogenic medium). Figure S4, Immunodetection of cardiac troponin T and PPARγ after 21 days of hMSCs culture (14 days in normal growth medium +7 days in adipogenic medium). Figure S5, Morphology of trypsinized and re-cultured hMSCs (group 3) from patterned and unpatterned groups were displayed. Figure S6, Validation of myocardial lineage commitment of trypsinized and re-cultured hMSCs using cardiac troponin T marker (re-cultured in normal growth medium, osteogenic and adipogenic induction medium respectively). Figure S7, Investigation of tissue-lineage commitment of hMSCs grown in osteogenic and adipogenic induction media for 2 weeks. Figure S8, Observing the morphological differences in hMSCs from both patterned and unpatterned groups. (DOC)