GDH dsRNA has a specific, statistically significant silencing effect on GDH protein levels in WSSV-infected shrimp.

(A)Western blotting results at 24 hpi for 3–4 samples of gill tissue taken from WSSV-infected shrimp that were treated with the indicated dsRNA (or PBS vehicle only control) 3 days prior to challenge. A commercial GDH antibody (Proteintech) was used as the probe. Signal strength was quantified using ImageJ software, normalized relative to β-actin, and then expressed relative to the positive control, which was set to 1. (B) Aggregated data and statistical analysis of the results shown in (A). (DOCX)