Gene expression profiling of alveolar macrophages from normal lung and metastasis-associated macrophages

To profile the gene expression of macrophages associated with lung metastases and identify mechanisms by which macrophages regulate metasetatic growth Overall design: Lung metastases were induced by tail vein injection of syngenic mouse mammary tumor cells, dissected and processed in parallel with normal lungs for FACS sorting for the CD11B+ and CD11C+ subsets of CD45+;F4/80+;LY6C-;MRC1+;FSC hi cells. RNA from these cells was analyzed by RNA-seq.