Transcriptome-wide impact of food-restriction on the medio-basal hypothalamus of the ewe.

Here we used RNAseq to compare the transcriptomes of ewes fed ad libitum VS food-restricted ewes (hereafter AL and RES, respectively). Body Condition Score was used as an index to validate the impact of food restriction. The study was performed in adult ewes of the Ile-de-France breed. The experiment included 30 ewes in each group (ad lib and food-restricted) and was repeated the following year giving a grand total of 60 ewes fed ad lib and 60 food-restricted ewes. RNA was extracted from each ewe. Then, for each condition four random equimolar mixes of mRNA from 15 ewes were used for RNAseq --> 4 biological replicates.