Effects of HIST1H2ac knockdown on the DNA methylation landscape of breast cancer cell line MCF-7. Effects of HIST1H2ac knockdown on the DNA methylation landscape of breast cancer cell line MCF-7

There are 64 replication-dependent histone genes with different isotypes associated with each class of the five histone types, H1, H2a, H2b, H3 and H4 in the human genome. In a previous report, we have showed that HIST1H2ac serves as a master regulator of ERalpha-dependent gene activation via ERalpha recruitment, and mediates chromatin looping of regulatory elements of estrogen receptor-targeted genes. Here, we performed whole genome bisulfite sequencing of MCF-7 upon suppressing H2ac expression through siRNA transfection using two different siRNAs to determine the knockdown effect on DNA methylation. Our analysis showed the DNA methylation of ~99% of the genome were mostly unchanged comparing to normal MCF-7 (methylation levels difference within 0.2).