Mouse cardiac outflow tract single-cell RNA-seq

We have performed large-scale single-cell transcriptomic sequencing of mouse cardiac outflow tract, a major hotspot for human congenital heart diseases, at different development stages. We sought to dissect the cellular diversity (cellulome) of developing OFT, and provide gene signatures for each cell types/subtypes. Moveover, we tried to reconstruct the developmental trajectory of interested biological processes, e.g. EndoMT, and to identify the gene regulatory network based on the single-cell RNA-seq data.