Deficiency of RPSA reduces binding of P65 to proinflammatory cytokines promoter regions in HSV-1 infection

After HSV-1 infection, ribosomal protein RPSA can recognize viral DNA and promotes the expression of proinflammatory cytokines. Through ChIP-seq, we found that the enrichment of P65 in proinflammatory cytokines(Il1b, Il6, Il12b, etc.) promoter regions was decreased due to Rpsa deficiency. We constructed Rpsa induced konckout RAW264.7 cell lines and performed chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for P65 in WT and Rpsa-iKO RAW264.7 cells infected with or without HSV-1.