miRNA differential expression and miRNA-mRNA network construction in mice with acute pancreatitis

Acute pancreatitis (AP) is an inflammatory disease of pancreatic tissue caused by pancreatic acinar cell injury. However,its miRNA expression is not clear.A mouse model of acute pancreatitis was established by the establishment of Caerulein and LPS, high throughput miRNA sequencing was performed on the pancreatic tissues of mice in the normal group and model group. We found 25 miRNAs with relatively significant differential expression by analyzing the miRNA expression profile. Then we performed downstream target gene prediction for differentially expressed miRNAs. Analysising of KEGG, GO, GSEA and network interaction, it was found that its therapeutic effect was closely related to apoptosis, inflammatory response and autophagy process. Overall design: Wild-type C57BL/6 mice were randomly divided into two groups. Each mouse was given once per hour for 7 intraperitoneal injections.Model group were injected 50 mg/kg caerulein,and LPS was added for the last time. Control group was injected with the same amount of normal saline.Pancreatic tissue was taken for high throughput sequencing of mRNA 24 hours after the start of modeling.