Region specific astrocyte response to mutant GFAP in Alexander disease model mice during postnatal development.

Gene expression analysis was performed in Gfap+/+, Gfap+/R236H, and mGFAPTg170-2 transgenic mice, using the Aldh1l1-eGFP-L10a transgenic line (JD130) to isolate translating ribosomes and purify astrocyte specific transcripts for RNAseq. Overall design: Hippocampus and corpus callosum were collected from male mice at P14 in an FVBB6F1 background. Translating ribosomes were isolated using antibodies against EGFP to precipitate the EGFP-L10a fusion protein and the associated RNA purified for sequencing and gene expression analysis. N = 3 for each genotype with each sample consisting of tissues pooled from 5 mice (15 mice total).