Semi quantitative real-time PCR. The bacterial culture was induced with 0.2% L-arabinose and harvested after 3 hours of induction. The relative intensity of all the bands were quantified using ImageJ
Sequences of primers and probes used for Real-Time PCR. Sequences related to gene type X collagen and MMP13 are proprietary to Applied Biosystems Inc. and not disclosed.
Comparisons of the estimated sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy values after latent class analysis comparing Kato-Katz (KK) and, H