Antigen-binding kinetic parameters of the different Nb_An05 mutants as well as the wild type Nb_An05, Nb_An46 and Nb_An33 were determined using surface plasmon resonance (CM5/VSG-chip).
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The kon, koff and KD values and percentages trypanotolytic activity are shown for each clone. Percentage trypanolysis was determined relative to that of wild type (WT) Nb_An05 (set at 100%) and data are representative of two independent experiments. For each of these experiments 2×105 parasites were incubated with 1 µg Nb (VSG∶Nb molar ratio of 1∶20).
创建时间：
2015-12-02
